How to Combine High and Low Voltages in a Single Design ?

Hi,

In our Project, we are using Input Voltage 480V line having Common mode choke, differential noise removal and filter. We are generating Voltage 24V @ 2A through Flyback circuit (Topology). From 24V ---> 5V ---->3.3V ------> 1.5V -----> 1.1V. The low voltage signals are generated from PMIC and LDO.

Questions :

1. PCB Layout guidelines for combining the High voltage AC and DC in a single board? 

2. What are the challenges and how to overcome challenges involved in " PCB Layout guidelines for combining the High voltage AC and DC in a single board"?  

3. How to isolate the High Voltage and Low voltage rails in Board? The High voltage AC should add noise and disturb the Digital lines

4. How to choose the Stack up of PCB for the Merging High Voltage and DC Signal board?