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Internal calibration of AD7731


I have read through the datasheet and, I am not sure if the AD7731 should be disconnected from the analog signal when performing internal zero scale and internal full scale calibration.

For example, channels 5 and 6 in full differential mode, AIN- driven with a 2.5v voltage source (LM4140) and AIN+ driven from a custom sensor through an OpAmp. REF_in- is tied to ground and REF_IN+ is 5v

Should I disconnect the 2.5v and the sensor OpAmp from the device for calibration?

Should I disconnect only the sensor OpAmp?

Should I not disconnect anything for calibration?

Thanks for your input.