I want to use the AD8553 with a single supply of 5Vdc. My input swings from 0.06 to 0.131Vdc. Nothing Negative or below ground. I don't quite understand the datasheet of why I can't connect Vref to ground. The datasheet says for single supply applications tie this to a voltage between ground and Vcc and suggest this is 2.5Vdc or use a reference. I would like to apply gain and get as much usable range on Vout as possible. Maybe the max Vout of 4.8 (0.131 x ~36 for gain). Couldn't I just set Vref to ground(to save using a precision reference) or 0.1Vdc at a minimum (with a reference to achieve max usable range on Vout)? This is a low frequency application and the source is low impedance ~100ohms. I do see the lower Vout range (Vsat low maybe?) is 0.075Vdc. Thank you for your help.