pluto sdr standalone

If you are just trying to run an M file, that isn't possible since MATLAB itself won't run on a Pluto. If you mean something else please provide more detail on exactly what you are trying to do.

-Travis

just try to  m. file but if it isnt' possible do you have a different suggestion (for example is it possible to execute py. file (python program file or script)  run automatically at start from an USB  using the OTG functionality of the Adalm Pluto. ( try to execute program without using PC )  

The easiest possible option is to use C. See example here: https://wiki.analog.com/university/tools/pluto/devs/embedded_code

https://wiki.analog.com/university/tools/pluto/devs/usb_otg

To use python you would need to compile python itself for Pluto. We have no guides on how to do this.

-Travis